Posts

Describe the purpose of the methods used to achieve the outcomes of the project.

/in /by

LOOKING FOR PRACTICAL HELPER FOR MOLECULAR BIOLOGY TECHNIQUES

Please check out the attachments. I’ve got all the results

Document Preview:

Molecular Techniques (SLE321) Practical Report Section Mark INTRODUCTION and AIM (1 page max) -What is yZnt? -Clear aims and/or objectives o Overall aim(s) o Brief description of techniques used (1-2 sentences) o Here we…. ? PCR ? Ligate linear plasmid & PCR fragment ? Transform into E. coli ? Confirm by Taq pol. screen (colonies) and Southern blot (gel). -Hypothesis and expected outcomes o Expected band sizes in gels o Blue/white screening result o Expected bands in Southern blot /15 MATERIALS and METHODS (2 pages max) -Appropriate use of headings (Some of the headings in the lab manual can be appropriated) -Clear and concise descriptions of changes or alterations made to methods -Outline the methods used in paragraph form – not as the recipe format in the lab manual. Two scientific papers have been provided as examples (Heras et al, 2009) o PCR & visualization of product o Mini-prep o RE digest & AP treatment o Gel purification o Ligation o Heat-shock transformation o Southern blot o PCR colony screen There should be sufficient detail for another scientist to repeat the experiment. As you see from the papers, research scientists tend to write abbreviated methods when standard practices are employed. This is very field-specific. Your supervisor will be able to guide you when you publish your own research. As the prac write-up is a learning exercise, it is better to err on the side of too much rather than too little detail. /10 RESULTS (2 pages max) -Clear and concise description of results achieved with reference to methods. -Appropriate use of figures and figure legends o Gels should be clearly labeled (MWM, lanes, band sizes) o Photos of blue/white screen plates are not necessary o Use line diagrams where appropriate. o Interpretation of gel pictures and Southern membrane /25 DISCUSSION (1-2 pages) -Describe the purpose of the methods used to achieve the outcomes of the project. -Clear explanations when problems or errors were encountered with experiments…

Examine cellular interactions within tissues and between organ systems in order to recognize factors that contribute to mental and physical well-being

/in /by

SARCOPENIA SCHOLARLY PAPER-BIOLOGY OF AGING

The paper information is on the attached document. Also the PDF file is the article needed for the paper. Paperlengthbetween 2500-3000 words.

Document Preview:

Applied Final Project – Scholarly Paper
Subject: Sarcopenia
Scholarly Paper Subject Article: Sarcopenia, obesity, and natural killer cell immune senescence in aging: Altered cytokine levels as a common mechanism (Attached as PDF)
In this assignment, you are required to write a scholarly paper that summarizes a particular aspect of aging research. The final paper will need to be ~7 double spaced pages (approximately 2500-3000 words), excluding title and bibliographic pages.  This is a scholarly paper about a review article (a scientific paper, written for scientists that reviews what is currently known about a subject) or a research article (generally the result of a single (highly significant) experiment in series of related experiments).
You will be required to include the following sections:
1)      The importance of the subject to aging research in general
2)      An analysis of the article such that fellow class members will understand it without additional research.
3)      Information on how the mechanism of the condition discussed contributes to aging
4)      How the information revealed in the article may lead to treatments or cures
Within in those section you much address the following course outcomes:
Demonstrate an understanding of cellular structure and function in order to define homeostasis in aging
Examine cellular interactions within tissues and between organ systems in order to recognize factors that contribute to mental and physical well-being
Define the developmental processes involved in cellular maturation in order to make informed decisions about health maintenance and disease prevention throughout the human lifespan
Apply knowledge of human aging to influence decision making in economics and public health policy
Make sure to include a Reference section in which you provide the full citation of your chosen articles and any additional materials you used to prepare your paper; a full citations includes: title of the article,…

Explain how two right-handed people might have a left-handed child

/in /by

BIOLOGY ASSIGNMENT

HelpHemophilia is carried as an X-linked trait in
humans. There are a number of types of hemophilia, but in all of them, blood
fails to clot properly and even the slightest injury can be life-threatening. A
hemophiliac male (XhY) has children with a female (XHXh)
whose father was hemophiliac. What are the chances that any daughters will have
hemophilia or will carry the trait like their mother? What about their sons?

Document Preview:

Mendel’s pea plants exhibited a number of simple dominant/recessive traits. Let’s say a dominant homozygous purple –flowered plant is crossed with a white-flowered plant (recessive). Using P for the purple allele for flower color, and p for the white allele for flower color,
Write the genotypes for each of these two plants.
Purple flowered:
White-flowered:
What gametes might each plant produce?
Purple-flowered:
White-flowered:
If these two plants are crossed, give the genotypes and phenotypes of their offspring. (Use your paper to make a Punnett square, then give the results here.)
Phenotypes:
Genotypes:
Assume that right-handedness (R) is dominant to left-handedness (r) in humans.
What are the possible genotypes of a right-handed person? What is the genotype of a left-hander?
Right-hander:
Left-hander:
Explain how two right-handed people might have a left-handed child.
If a left-handed person who had one left-handed parent has children with a left-handed person, what predictions could you make about the handedness of their children?
In cattle, polled (hornless) (P) is dominant to horned (p). If a breeder of purebred cattle, all of which are polled, suspects that one of her prize bulls is heterozygous and carries the horned allele, how might she determine if her suspicion is correct?
In horses, trotter (T) is dominant over pacer (t). A trotter is mated to a pacer, and the foal they produce is a trotter. Give the genotypes of these three horses.
Trotter:
Pacer:
Foal:
In rabbits, black color is due to a dominant gene, B, and brown color is due to its recessive allele, b. Short hair is due to the dominant gene, S, while long hair is due to its recessive allele, s.
In a cross between a homozygous black, long-haired rabbit and a brown, homozygous short-haired one, describe the nature of the F1 generation. What is the phenotype of the resulting offspring? What is their genotype?
Phenotype:
Genotype:
What does the F2 generation look like?…

Bacterial cells are prokaryotic. Choose two structures of bacterial cells and explain how these structures allow bacteria to function as unicellular organisms.

/in /by

EMERGENCY BIOLOGY DUE TONIGHT

SCI206-0803A-15 Biology
Assignment Name: Unit 2 Discussion Board
Deliverable Length: 3-4 paragraphs
Details:There are two main types of cells: prokaryotic and eukaryotic.

1. Bacterial cells are prokaryotic. Choose two structures of bacterial cells and explain how these structures allow bacteria to function as unicellular organisms.
2. There are many examples of eurkaryotic cells. Algae, Fungi, Plants, and Animals are all composed of eurkaryotic cells. Some algae and fungi are unicellular organisms, but other algae and fungi, and all plants and animals are multicellular organisms that are composed of specialized eukaryotic cells that interact to support the life of the multicellular organism.

Choose either a plant or an animal cell. Describe two structures of the cell and how these function to support both the function of the cell and overall life (function) of a plant or animal organism.

Objective: Explain the processes of photosynthesis and cellular respiration. Describe the relationship between chemistry and the characteristics of living things.

WRITE AN ARTICLE ON A MAJOR TOPIC WITHIN THE GENERAL FIELD OF MICROBIOLOGY

/in /by

WRITE AN ARTICLE ON A MAJOR TOPIC WITHIN THE GENERAL FIELD OF MICROBIOLOGY.

You are the science reporter for your local newspaper. Your editor asks you to write an article on a major topic within the general field of microbiology. He stipulates that in this article you need to include the following three things:

  1. An historical timeline for the major scientific discoveries that has led to our current understanding of the topic.
  2. A summary of our current understanding of the topic.
  3. A projection of where the next major advance or breakthrough in your topic will come from.

Description

Using the topic, outline and resources that you developed for Project 1, write a 2,000 word article for your newspaper following your editor’s instructions. Your article should not just quote the research articles you have consulted, but convert them into language that is understood by non-scientists without losing the meaning.

Procedure

  • Submit your project in one WORD document and place it in the appropriate assignment dropbox.
  • Please format your article according to APA style. Your article should have a cover page, abstract, and reference page. These pages do not count towards the length specified (2000 words).

 

  • Please number each page, including the cover page and provide a running head.

What is the difference between Microbiology and Clinical Microbiology?

/in /by

INTRODUCTION TO MICROBIOLOGY
Case Assignment
For your first Case Assignment, IN YOUR OWN WORDS, answer these questions reviewing the content in your Course Overview within the Syllabus:
1. What will MIC 100 emphasize?
2. Why is microbiology important to health professionals?
3. How does death by infection compare to death by warfare?
4. What issues surrounding antibiotic resistance are named?
5. What STD epidemics are named?
6. What websites contain excellent resources for this class?
Now answer these questions regarding the material contained on the Module 1 Home Page:
1. Complete this sentence: Infections have killed more humans than…
2. What is the difference between Microbiology and Clinical Microbiology?
3. Name the three defense mechanisms listed.
4. What is a pathogen?
5. What Host-Microbe relations are described?
6. Where do you find the assignments for all of your SLPs?
Cells are the smallest unit of life. All cells are bound by a cell membrane which separates the internal parts of the cell from the external environment.
For the final part of this Case assignment, describe cell theory (hint: there are three components). Briefly describe the work of two scientists that contributed to this theory. Be sure to include your references for this answer.
Watch this video about your cells and answer these questions:
http://www.pearsoncustom.com/mct-comprehensive/asset.php?isbn=1269879944&id=8524
What does the video say is the function of:
• Cytoskeleton
• mictochondria
• DNA
Note: The assignment will require you to engage in independent research of cell theory. You will find information on scientists who contributed to this theory in your required reading for your SLP. You will also find additional resources on your ebrary bookshelf, such as the book, Cell Biology: Fundamentals and Applications, a great place to begin your investigation of cell theory and cell biology.
Your assignment is due by the Monday following the end of this module. Please upload your Case Assignment.
Assignment Expectations
Please include a cover sheet for your Case Assignment. Use headings to separate each section’s questions, and answer each question using complete sentences.
References: List the references you used to describe cell theory. Cite them within your text (Author, date) and provide the full reference information in a References section.
Grammar and Spelling: While no points are deducted, assignments are expected to adhere to standard guidelines of grammar, spelling, punctuation, and sentence syntax. Points may be deducted if grammar and spelling impact clarity.
The following items will be assessed:
Assignment-Driven Criteria – Did you address each requirement?
Critical Thinking – How well did you synthesize and evaluate the topics addressed?
Scholarly Writing – Is everything explained in complete sentences?
Quality of References and Assignment Organization – Did you organize your paper with headings?
Citing Sources – Did you list your references and cite information where necessary?

How does microbial metabolism contribute to biogeochemical cycles?

/in /by

Microbiology is one of the fundamental areas of knowledge for the health professions. We must know how to identify microorganisms in our environment and how to prevent and control the numerous pathogens that infect virtually everyone on our planet at one time or another. Once we understand the etiology of an infectious disease we are able to not only cure infected persons from the various pathogens found in our environment, but prevent others from becoming infected.
We will begin this course with a review of the history of microbiology and an introduction to cell theory. You will learn how the work of scientists Robert Hooke, Robert Koch, Louis Pasteur, and several others, led to the controversy over spontaneous generation and the understanding of the role of microorganisms in the causation of disease. The work of these pioneers in the field ultimately contributed to the development of cell theory and medical microbiology.
Once you understand the history of microbiology and cell theory, we will learn to identify different kinds of microorganisms and classify them based on certain characteristics. We will consider the concepts of how we classify “species” and how species evolve, as we learn about the classification of microbes into groups such as bacteria, fungi, protozoa, and viruses.
We will then build on this general understanding of microbial biology to examine how our bodies defend against infection and disease, and explore modern concepts of disease control and prevention. In this section we will focus on some current issues surrounding antibiotic resistance (MRSA, VRSA) and STD epidemics facing the human population, such as HIV and HPV, and current attempts to control them. In order to understand why these microbes pose such a risk to our population, we will learn the basics of the human immune response, immunity, and how vaccinations and antibiotics play a role in disease control and prevention.
As we investigate these basic theories of microbiology, we will consider how the human microbiome plays a role in health and disease, and consider the microbiome found in our soil and water. We will address questions such as:
• What are the resident microbiota? What is the difference between a nonpathogenic compared to a pathogenic microbe? What conditions can cause a nonpathogenic microbe to become pathogenic?
• What is metabolism and how do microbes differ in their ability to convert energy into usable forms?
• How does microbial metabolism contribute to biogeochemical cycles?
You will begin each module by completely reading the Home page. The Case Assignment will build on the material contained in the Home page and you will accomplish readings and quizzes in the Boundless Textbook for Microbiology for your SLP assignments. Many additional resources are available to you on your ebrary bookshelf for Microbiology. You may use these to help you accomplish any research for your Case Assignments. Please use the CDC and WHO websites as well as university sites, as these are considered academic sources.
Remember, as you complete your assignments, it is important to clearly document all work submitted in the course. Work should include a title page documenting student’s name, type of assignment (Case or SLP), instructor’s name and the date. All research should be synthesized and presented as ORIGINAL WORK (use your own words!). Adhering to these practices will insure that proper credit is given for each submission. Once again, welcome to Microbiology.

WHY IS MICROBIOLOGY IMPORTANT TO HEALTH PROFESSIONALS?

/in /by

WHY IS MICROBIOLOGY IMPORTANT TO HEALTH PROFESSIONALS?

4 pages

Apa

Sources:4

Additional Resources:
Singh, U. S. and Kapoor, K. “Introduction to Microbiology,” and “Microbial Cell Structure,” in Introductory Microbiology, 2010. Global Media, Jaipur IND. 2010. eISBN: 9789350431238
Mishra and Agrawal. “Chapter 1: Introduction,” in A Concise Manual of Pathogentic Microbiology, Wiley, Somerset NJ. 2013. eISBN: 9781118301210
Course Overview
Welcome to MIC 100 – Microbiology! This is a fascinating course designed to give students an overview of the microbial world, the beneficial aspects of microbes, and the role of microbes in human disease. We will emphasize the public health aspects of infectious disease, mode of transmission of disease, pathogenicity, inducible host resistance, antigens and antibodies, disease prevention, and the principles of microbial control.
Did you know that during the past century alone more than 500 million people have died of infectious diseases and nearly 5 billion have suffered from debilitating infectious diseases? Compare this number to the less than 100 million people who have died as a result of war-related casualties in the past 500 years. (Mishra and Agrawal 2013).

Identifies light and electron as two main branches of Microscopes

/in /by

LIST IMPORTANT DISCOVERIES IN MICROBIOLOGY AND THEIR IMPORTANCE

Unit outcomes addressed in this Assignment:

  • List important discoveries in microbiology and their importance
  • Discuss the classification schema
  • Select appropriate microscopic method to study different types of microorganisms

 

Instructions

  • In an essay, describe the various mechanisms utilized within the field of microscopy for studying microbes.
  • Be sure to include the appropriate staining techniques.

 

Requirements

  • Your essay should be a minimum of 500 words
  • Be sure that your grammar, sentence structure, and word usage is appropriate.
  • APA FORMAT

 

Guidelines

  • Identifies light and electron as two main branches of Microscopes
  • Identifies functional differences between the two main branches of microscopes
  • Identifies the differences in staining techniques
  • Provides specific microbial staining examples
  • Identifies how microscopy is utilized in identifying unknown

microbial specimen.

Discuss the positive or negative aspects of these drug interventions or solutions.

/in /by
    • Case Studies

       

      Anorexia

       

      Beth is a normal child raised in a well-balanced home by caring parents. As a teenager, she began to experience an overwhelming fear of gaining weight and becoming fat. Her fear was unfounded because Beth’s weight was normal for her height and age. Beth began to diet and lose weight, but regardless of how much she weighed, she had a very poor self-image. Beth has become dangerously thin, but she denies the seriousness of her condition. Regardless of how much weight she loses, she feels like she needs to lose more. Beth has missed several menstrual cycles and continues to severely restrict her food intake. Her weight continues to drop. Beth’s mother and father are deeply concerned, but they do not know how to help their daughter (PsychCentral®, 2006).

       

      Drug Abuse

       

      Ron is a 33-year-old man who has been in and out of the court/jail system for the last several years. He started drinking as a teenager but his alcohol abuse began to be a serious problem in his late 20s. Ron has several DUI’s (driving under the influence) and has been arrested several times, but he seems unable to control his drinking. To his credit, he admits that he is an alcoholic. Ron has been through a variety of inpatient treatment facilities for his alcoholism, but after a brief time of sobriety, he has always relapsed back into his daily abuse of alcohol. His wife is concerned about him, but she does not know what to do for her husband.

       

      Anxiety

       

      Tom is an engineer, he is happily married, and he is the father of three bright, healthy children. By all appearances, his life is stable and satisfying. Tom, however, suffers from continual worry that he has a difficult time turning off. His anxiety may center on anything from his perceived health problems (he has recently been to his doctor for a physical, but no health issues were discovered) to money and job responsibilities. At times his anxiety peaks to the point that it interferes with his ability to function on the job. Physical symptoms include muscle tension, headaches, and hot flashes that often accompany Tom’s anxiety. Tom often feels nauseated, and he becomes easily fatigued. When he feels anxious, Tom has difficulty concentrating, he becomes irritable, and he has difficulty falling asleep at night. All of these symptoms have been present for the last 6 months. Tom has tried to talk himself out of his anxiety, but this has not worked for him. Tom’s wife is supportive, but she does not know what to do for her husband (Hauser, 2005).

       

      Insomnia

       

      Mary is a single mother of one child. She has had difficulty sleeping for the last month. Her lack of sleep has caused her to be fatigued during the day, which has caused significant impairment in her professional and social life. Mary has no history of mental disorders (such as depression) nor is she on any kind of prescription medication. Mary does not drink alcohol and does not take any type of street drug. Mary has been to her doctor about this problem, but he has been reluctant to give her any kind of sleep medication for her insomnia because he is concerned about her becoming overly dependant on the medication. Mary is facing yet another night with little sleep.

       

      References

       

      Hauser, J. (2005, February 2). Anxiety: Generalized anxiety disorder. Retrieved November 21, 2006, from the PsychCentral® Web site: http://psychcentral.com/disorders/anxiety/gad.html

       

      PsychCentral® (2006, November 17). Anorexia nervosa: Symptoms. Retrieved November 21, 2006, from http://psychcentral.com/disorders/sx2.htm

       

    • Discuss the positive or negative aspects of these drug interventions or solutions.
  • Conclusion