A solution of BSA is made up by weighing out 20 mg of the lyophilized (freeze dried) powder of the protein supplied and dissolving in 2 mL water.

BSA made up weighing out mg the lyophilized (freeze dried) powder the protein supplied dissolving mL water. The concentration the protein was checked adding mL the solution to mL water; the A280 the resulting solution relative to a water blank was . The absorption coefficient BSA (mg mL cm”‘. Calculate the actual concentration of the original solution of BSA and comment on your answer.